Knowledge base

Here in America and in many other parts of the world, hematoxylin-eosin staining is used as a routine stain for all tissues.

But in a French-speaking village in Quebec, some pathologists are valiantly resisting the invader and continue to use hematoxylin-phloxin-safran staining as a routine stain.

Yet the issue has already been studied! G. Silvestrini and associates published a study in Rome in 2002, in which they dared to try switching from absolute to denatured ethanol, Here's an extract from their publication.

The development of the stains we use routinely today took place over long periods of time, with a lot of trial and error, mostly empirically, and thanks to the contributions of many researchers. Nowhere is this truer than in the case of hematological staining of blood elements.

Unless you know histology, and even if you do, this title might be difficult to understand!

Discovered at the end of the 19th century, this Mexican tree extract stains only when oxidized (naturally by aging or chemically) and coupled with a metallic mordant.