Keyword: Tissue processing
Too much is like not enough
After fixation in histology tissue processing, comes the second step; dehydration. Water has to be removed from the tissue to render it miscible with paraffin, to make blocks that can be cut on the microtome and then stained.
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Clear as a bell, as my father used to say.
Today, we're going to talk about clarification, a process that is part of histology tissue processing and typically achieved with a solvent.
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XYLENE VS TOLUENE
I learned lately that Europeans don’t know that toluene can be used for clarification in histology and they strictly use Xylene.
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